HuagongsheHuagongshe Chemie-Druckbericht · Nur für Forschungszwecke
7-Amino-4-methylcoumarin-3-acetic acid
AMCA-H · AMCA blue · AMCA acid · AMCA (coumarin) · AMCA Free Acid (Blue) · 2H-1-Benzopyran-3-acetic acid · AMCA acid *CAS#: 106562-32-7* · AMINO-METHYL-COUMARINACETICACID
Identifikatoren & physikalische Daten
| CAS No. | 106562-32-7 |
|---|---|
| Summenformel | C12H11NO4 |
| Durchschnittliche Molmasse | 233,22 g/mol |
| Monoisotopische Masse | 233,06880783 g/mol |
| SMILES | Cc1c(CC(=O)O)c(=O)oc2cc(N)ccc12 |
| InChIKey | QEQDLKUMPUDNPG-UHFFFAOYSA-N |
| PubChem CID | 129367 |
| EC No. | 635-163-2 |
| DTXSID | DTXSID20147648 |
Chemische & physikalische Eigenschaften
| XLogP | 0,6 |
|---|---|
| Polarare Oberfläche (TPSA) | 89,6 |
| H-Brücken-Donoren | 2 |
| H-Brücken-Akzeptoren | 5 |
| Frei drehbare Bindungen | 2 |
| Schwere Atome | 17 |
| Formalladung | 0 |
| Komplexität | 388 |
| Stoffklassen | Dyes -> Coumarin Dyes |
| Physical Description | Light brown powder[Sigma-Aldrich MSDS] |
| Siedepunkt | 512.2±50.0 °C(Predicted) |
| Dichte | 1.391±0.06 g/cm3 (20 ºC 760 Torr) |
| Lagerbedingungen | Keep in dark place,Sealed in dry,Room Temperature |
| Löslichkeit | DMF: soluble |
| Aggregatzustand | powder to crystal |
| pKa (Säurekonstante) | 3.80±0.10(Predicted) |
| Farbe | Light yellow to Brown |
| BRN | 7927205 |
| InChI | InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15) |
| InChIKey | QEQDLKUMPUDNPG-UHFFFAOYSA-N |
| SMILES | C1(=O)OC2=CC(N)=CC=C2C(C)=C1CC(O)=O |
Sicherheit & Vorschriften
| GHS-Einstufung | WarningH315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 39 reports by companies from 2 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. |
|---|---|
| Gefahrenübersicht | An irritant[Sigma-Aldrich MSDS] See Coumarin 120. |
| Hazard Classes and Categories | Skin Irrit. 2 (100%) |
| Wassergefährdungsklasse (WGK) | 3 |
| Zollcode | 29322090 |
| R-Sätze (R) | 36/37/38 |
| Gefahrensymbole | Xi |
| Sicherheitshinweise (S) | 26-36 |
| Lagerklasse | 11 - Combustible Solids |
| Gefahrenklasse | Eye Irrit. 2Skin Irrit. 2STOT SE 3 |
Anwendungen & Verwendung
Beschreibung
AMCA is fluorescent protein labeling agent. It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. It has commonly been used in multiplex immunophenotyping applications.
Verwenden
7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is a blue fluorescent dye. Its desirable properties include a relatively large Stoke's shift and resistance to photobleaching. 7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is used as contrasting probes for double and triple labeling in im