1-Ethyl-3-(3'-dimethylaminopropyl)carbodiimide → HCID 44253628, Verbindung

Beteiligte Stoffe

Reaktionsbedingungen

Reaktionsschritte

A solution of (S)-3-cyclohexyl-2-[4-(2,3-dihydro-benzo[1,4]dioxin-5-yloxy)-2-oxo-2,5-dihydro-pyrrol-1-yl]-propionic acid (200 mg, 0.52 mmol) in dichloromethane (10 mL) was treated with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (81 mg, 0.52 mmol) and 1-hydroxybenzotriazole (75 mg, 0.54 mmol). The reaction mixture was stirred at 25° C. for 2 h followed by the addition of 1-(3-amino-pyrazol-1-yl)-2-methyl-propan-2-ol (prepared in U.S. Pat. Appl. US2008021032 Example 80, 88 mg, 0.57 mmol). The reaction mixture was stirred for 18 h at 25° C., under nitrogen. The reaction mixture was diluted with dichloromethane, washed with 2N aqueous hydrochloric acid, saturated sodium bicarbonate solution, a saturated sodium chloride solution and dried over magnesium sulfate. The organic layer was concentrated, and the crude product was purified by ISCO flash chromatography (Teledyne Isco RediSep Flash Column 12 g; 0% to 100% ethyl acetate/hexanes) to afford (S)-3-cyclohexyl-2-[4-(2,3-dihydro-benzo[1,4]dioxin-5-yloxy)-2-oxo-2,5-dihydro-pyrrol-1-yl]-N-[1-(2-hydroxy-2-methyl-propyl)-1H-pyrazol-3-yl]-propionamide (117 mg, 43%) as a white solid: LR-ES-MS m/z calculated for C28H36N4O6 [M]+ 524, observed [M+H]+ 525; 1H NMR (300 MHz, DMSO-d6) δ ppm 0.74-1.33 (m, 6H) 1.04 (s, 3H) 1.06 (s, 3H) 1.49-1.89 (m, 7H) 3.89 (s, 2H) 4.11 (d, J=18.4 Hz, 1H) 4.28 (s, 4H) 4.52 (d, J=18.4 Hz, 1H) 4.68 (s, 1H) 4.84 (s, 1H) 4.89 (dd, J=10.6, 4.8 Hz, 1H) 6.44 (d, J=2.1 Hz, 1H) 6.72-6.95 (m, 3H) 7.53 (d, J=2.1 Hz, 1H) 10.77 (s, 1H).

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