Dicyclohexylcarbodiimide → N,N'-Dicyclohexylurea

Beteiligte Stoffe

Reaktionsschritte

To a stirred solution of 5- or 6-carboxyfluorescein (0.5 mmol) in dry DMF (5 mL) were added N-hydroxysuccinimide (1.1 equiv.) and 1,3-dicylcohexylcarbodiimide (1.1 equiv.). After about 10 minutes, a white solid (dicyclohexylurea) started forming. The reaction mixture was stirred under nitrogen at room temperature overnight. TLC (thin layer chromatography; 9:1 CH2Cl2—MeOH) indicated complete disappearance of the starting material.

Herkunft & Nachweise