Elastase (human leucocytes)

UsesElastase from human leukocytes has been used: to measure serum elastase activityin proteolytic digestion of fibronectin and salivary glandsin neutrophil elastase (NE) activity assaycell-free NE digestion of E-cadherinscratch wound assayin a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal modelsElastase has been used to digest fibronectin. The results were compared with fibronectin digestion by crude human leukocyte homogenate to examine the presence of fibronectin peptides in saliva of patients with Sjgren′s syndrome. It has also been used as a reference to determine the elastase activity in cell lysates. This study examined the effect of all-trans retinoic acid on procoagulant and fibrinolytic activities of cultured blast cells. These blast cells were from patients with acute promyelocytic leukemia.

General DescriptionElastase is a proteolytic enzyme. It is a member of the subgroup named, peptidyl peptide hydrolases. It is a major anatomic constituent of arteries. It is mainly found in the pancreas and pancreatic juice of various birds and mammals. It is also present in human serum, granulocytes and erythrocytes.