Tetramethylammonium hydroxide → 2Alpha,3alpha-epoxy-16beta-(1-pyrrolidinyl)-5alpha-androstan-17beta-OL

Participants

Reaction Steps

Analysis of rocuronium bromide, Compound I, was performed with a column & packing Hypersil silica; 5 μm, 250 mm×4.6 mm, Cat No. 30005-254630 or equivalent. Eluent A was tetramethylammonium hydroxide Buffer, 0.05M, pH 7.4, eluent B was acetonitrile, and the composition was 10:90, A:B. The stop time was 25 min., the flow rate was 2.0 mL/min., the detector was UV at 210 mn, the column temperature was 30° C., and the injection volume was 5 μl.

Provenance & Evidence