化工社化工社 化学情報印刷レポート · 研究参考用
Distearoylphosphatidylethanolamine → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Distearoylphosphatidylethanolamine | HCID 102547 | — |
| 反応物 | 1,2-Bis(dimethylphosphino)ethane | HCID 141059 | — |
| 反応物 | Tesmilifene Hydrochloride | HCID 175534 | — |
| 反応物 | 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine | HCID 445468 | — |
| 反応物 | 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine | HCID 447078 | — |
| 反応物 | 1,2-Dilauroyl-sn-glycero-3-phosphoethanolamine | HCID 9546801 | — |
| 反応物 | [(2R)-3-[2-aminoethoxy(hydroxy)phosphoryl]oxy-2-tetradecanoyloxypropyl] tetradecanoate | HCID 9852308 | — |
| 溶媒 | Chloroform | HCID 6212 | — |
| 溶媒 | Dimethylformamide | HCID 6228 | — |
| 生成物 | 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid | HCID 3383 | — |
反応手順
PE lipids (1,2-dilauroyl-sn-glycero-3-phosphoethanolamine, DMPE; 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine, DMPE; 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, DPPE; 1,2-dioctadecanoyl-sn-glycero-3-phosphoethanolamine, DSPE, Avanti polar lipids. Inc.) were dissolved in 500 uL CHCl3 and 500 uL DMF, 1.2 eq of fluorescein-PEG2000-NHS (creative PEG works Inc.) was added and the reaction mixture were agitated overnight, the amphiphilic fluorescein PEG amphiphiles were purified by reverse phase HPLC using a C4 column (BioBasic-4, 200 mm×4.6 mm, Thermo Scientific), 100 mM triethylamine-acetic acid buffer (TEAA, pH 7.5)-methanol (0-30 min, 10-100%) as an eluent.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US09107904B2 |
| 出典データセット | uspto-grants-2015_08 |
| ORD ID | ord-f710ef369470408c8e0c9f4e03abaeff |
| 出典メモ | uspto-grants-2015_08 |