関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Fmoc-L-tryptophan | HCID 978343 | — |
| 反応物 | HCID 178427573 | HCID 178427573 | — |
| 溶媒 | Trifluoroacetic acid | HCID 6422 | — |
| 生成物 | Fmoc-Trp(2Pmc)-OH | HCID 131724867 | — |
反応手順
Fmoc-Arg(Pmc)-OH (0.5 g, 0.75 mmol) and Fmoc-Trp-OH (0.43 g, 0.1 mmol) was dissolved in 10 ml 100% TFA and heated at 30° C. for 1.5 hours. After evaporation of TFA, Fmoc-Arg-OH was removed by column chromatography on silica gel with heptane/ethyl acetate 2:1 as mobile phase. Fmoc-Trp(2Pmc)-OH was isolated by preparative HPLC (C18, 70-100% B in 15 min., tR 14.8 min, (A: H2O+0.1% TFA; B: CH3CN+0.1% TFA)). Isolated yield 130 mg (0.19 mmol, 25%).
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US09109048B2 |
|---|
| 出典データセット | uspto-grants-2015_08 |
|---|
| ORD ID | ord-5084c9e428ae4f7c91bf3eea7361e819 |
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| 出典メモ | uspto-grants-2015_08 |
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