1,4-Piperazinediethanesulfonic acid, potassium salt (1:2)
PIPES DIPOTASSIUM · PIPES 2K · PIPES-K2 · PIPES DIPOTASSIUM SALT · METHYLCELLULOSE 4000 CP · PIPES DIPOTASSIUM SALT, VETEC · PIPES dipotassiuM salt (PIPES-K{2}) · PIPES dipotassium salt, 99%, for bio
식별자 및 물리적 특성
| CAS No. | 108321-27-3 |
|---|---|
| 분자식 | C8H16K2N2O6S2 |
| 평균 분자량 | 378.6 g/mol |
| 단동위원소 질량 | 377.97239159 g/mol |
| SMILES | O=S(=O)([O-])CCN1CCN(CCS(=O)(=O)[O-])CC1.[K+].[K+] |
| InChIKey | BJQYFCAMUXGZFN-UHFFFAOYSA-L |
| PubChem CID | 176078 |
| DTXSID | DTXSID9073042 |
화학 및 물리적 성질
| 극성 표면적 | 138 |
|---|---|
| 수소 결합 공여자 수 | 0 |
| 수소 결합 수용자 수 | 8 |
| 회전 가능 결합 수 | 4 |
| 무거운 원자 수 | 20 |
| 형식 전하 | 0 |
| 복잡도 | 370 |
| 물질 분류 | Other Uses -> Biological Buffers |
| Physical Description | White solid[Sigma-Aldrich MSDS] |
| 저장 조건 | Store at RT. |
| 용해도 | H2O: 0.5 g/mL, 투명, 무색 |
| 물리적 상태 | crystalline powder |
| pH | 6.1-7.5 |
| pKa(산도 계수) | 6.8(at 25℃) |
| 용해도 | water: 0.5g/mL, clear, colorless |
| 주요 응용 | 진단 검사 제조 |
| InChI | 1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)/q2*+1/p-2 |
| InChIKey | BJQYFCAMUXGZFN-UHFFFAOYSA-L |
| SMILES | [K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O |
| EPA | 1,4-Piperazinediethanesulfonic acid, dipotassium salt (108321-27-3) |
화학적 성질
White crystalline powder
안전 및 규제
| 규제 정보 | PMN - indicates a commenced PMN (Pre-Manufacture Notices) substance. |
|---|---|
| 유해성 요약 | Not considered hazardous by GHS classification[Sigma-Aldrich MSDS] See PIPES. |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| 관세 코드 | 29335995 |
| 위험 문구(R) | 36/37/38 |
| 위험 표시 | Xi |
| 안전 문구(S) | 26-37/39 |
| F 고인화성물질 | 10 |
| 저장 분류 | 11 - Combustible Solids |
용도
용도
PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.
일반 설명
PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.