Phosphate → Phosphate

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1.0 M Phosphate buffer was prepared and 30 mL aliquots were adjusted to the following pHs: 3, 7, 10, and 12. 22.2 uL of 1000 ppm KATHON® 886 MW biocide was added to a semi micro cuvette followed by 2.065 mL of one of the above phosphate buffers. These preparations resulted in a final isothiazolinone concentration 10 ppm. The solution was mixed and allowed to stand for one minute before measuring the absorbance spectrum from 250 nm to 500 nm. Additional spectra were obtained until no notable change between subsequent spectra was observed.

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