Diisopropylethylamine → 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [(S)-1-(5-trifluoromethyl-1H-imidazol-2-yl)-ethyl]-amide

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To a stirred solution of 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid (40 mg, 90.6 μmol), (S)-1-(5-(trifluoromethyl)-1H-imidazol-2-yl)ethanamine hydrochloride (32.5 mg, 151 μmol), diisopropylethylamine (63.3 μL, 362 μmol) in DMF (2 mL) was added o-(benzotriazol-1-yl)-N,N,N′,N′-bis(tetramethylene)uronium hexafluorophosphate (43.0 mg, 99.7 μmol). After 15 hours the reaction mixture was diluted with dichloromethane and water. The organic layer was washed with saturated sodium carbonate and brine then dried (sodium sulfate), filtered and concentrated in vacuo. The residue was purified by chromatography (silica, 12 g Analogix column, 50-70% ethyl acetate in hexanes, gradient over 30 min) to give 2-(6-fluoro-1-methyl-1H-indazol-3-yl)-5-((2-(trimethylsilyl)ethoxy)methyl)-5H-pyrrolo[2,3-b]pyrazine-7-carboxylic acid [(S)-1-(5-trifluoromethyl-1H-imidazol-2-yl)-ethyl]-amide (34 mg, 62%) as a light yellow solid. LCMS, [M+H]=603.

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