Acetone → 25-Hydroxyvitamin D3

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Also, when a mass-production was carried out by varying the kinds of an organic solvent, ethanol showed a calcitriol production yield of 48.45 mg/L, and acetone and DMSO showed calcitriol production yields of 74.87 mg/L and 70.85 mg/L, respectively, and calcifediol production yields of 156.37 mg/L and 141.81 mg/L, respectively. Especially, methanol showed a calcitriol production yield of 90.12 mg/L, and a calcifediol production yield of 166.87 mg/L (see Example 9). Accordingly, it can be seen that the inventive composition including methanol, ethanol, acetone, and DMSO showed a high calcitriol or calcifediol productivity.

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