참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|
| 반응물 | Triethylamine | HCID 8471 | — |
| 반응물 | NHS-Biotin | HCID 434213 | — |
| 반응물 | N-(6-Aminohexyl)-4-azidobenzamide | HCID 13330101 | — |
| 용매 | Dimethylformamide | HCID 6228 | — |
| 생성물 | Biotin [2-(4-azidobenzoyl)aminohexyl]amidE | HCID 131721358 | — |
반응 단계
1.30 g (5 mmol) of product 2b are suspended in 40 ml of distilled DMF and 1.4 ml triethylamine are added under stirring at 20° C. Subsequently, 1.71 g (5 mmol) of biotin-N-hydroxysuccinimide ester 3 are added and stirring is continued for another 3 h at 20° C. Subsequently, the solvent is removed on a rotation evaporator (oil pump vacuum, water bath 50° C.) and the concentrated residue is digested overnight with saturated NaHCO3 solution. Product 4b is drawn off, washed with water and dried in the exsiccator.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 특허 번호 | US05952185 |
|---|
| 출처 데이터셋 | uspto-grants-1999_09 |
|---|
| ORD ID | ord-5565c67d56f746eb8a2c86c07a4205b6 |
|---|
| 출처 메모 | uspto-grants-1999_09 |
|---|
