反应详情

8-Bromo-2-[(2-methylpropyl)oxy]-9-(tetrahydro-2H-pyran-4-ylmethyl)-9H-purin-6-amine (100 mg) in n-butanol (2 mL) was refluxed with concentrated hydrochloric acid (2 mL) at 100° C. (external temperature) for 3 hours. The reaction mixture was evaporated under reduced pressure to dryness. To the reaction mixture was then added a small volume of water and an equivalent volume of methanol to give a suspension, which was then neutralised to by the addition of 2M sodium hydroxide (from pH ˜3 to 7). This was then evaporated under reduced pressure to give a beige solid which was purified by C18 reverse phase chromatography using water (containing 0.1% formic acid)-acetonitrile (containing 0.05% formic acid) as eluent (20-60%). This gave the title compound (13 mg) as white solid.

1. customThe reaction mixture was evaporated under reduced pressure to dryness
2. additionTo the reaction mixture was then added a small volume of water
3. customan equivalent volume of methanol to give a suspension, which
4. customThis was then evaporated under reduced pressure
5. customto give a beige solid which
6. customwas purified by C18 reverse phase chromatography