反应详情

3-[13-(2-Cyanophenylsulfonyloxy)-5-methylphenoxy]propoxy guanidine hydrochloride: To a solution of 3-[3-(2-cyanophenylsulfonyloxy)-5-methylphenoxy] propoxyamine (362 mg, 1.0 mmol), as prepared in the preceding step, in N,N-dimethylformamide (10 mL) was added 1H-pyrazole-carboxamidine hydrochloride (590 mg, 4.0 mmol). The reaction mixture was stirred at ambient temperature for two days. N,N-Dimethylformamide was removed under high vacuum. Acetonitrile (10 mL) was added, the solid was removed by filtration, the filtrate was concentrated in vacuo, and the residue was dried under high vacuum. The residue was partitioned between water (30 mL plus 2 mL brine) and diethyl ether (20 mL). The water solution was extracted with diethyl ether (20 mL), and the combined diethyl ether extracts were extracted with acidic water (pH 5). The combined water solutions were adjusted to pH 8-9 by using 2N NaOH and extracted with ethyl acetate (3×50 mL). The ethyl acetate solution was washed with pH 7 buffer solution (2×30 mL) and brine (30 mL) and dried over Na2SO4. After removing the solvent. 0.6 N HCl methanol (10 mL) was added, and the solution was concentrated to give the title compound as a colorless oil (340 mg, 77%). 1H-NMR (300 MHz, DMSO-d6) δ 8.30 (d, J=7.5 Hz, 1H), 8.09 (t, J=7.5 Hz, 1H), 8.04 (m, 2H), 7.72 (br s, 5H), 6.79 (s, 1H), 6.49 (s, 1H), 6.47 (s, 1H), 3.99 (t, J=6.2 Hz, 2H), 3.90 (t, J=6.3 Hz, 2H), 2.22 (s, 3H), 2.01 (m, 2H). Mass spectrum (MALDI-TOF, α-cyano-4-hydroxycinnamic acid matrix) calcd. for C18H20N4O5S: 405.1 (M+H), 427.1 (M+Na). Found: 405.1, 427.0.

1. customN,N-Dimethylformamide was removed under high vacuum
2. additionAcetonitrile (10 mL) was added
3. customthe solid was removed by filtration
4. concentrationthe filtrate was concentrated in vacuo
5. customthe residue was dried under high vacuum
6. customThe residue was partitioned between water (30 mL plus 2 mL brine) and diethyl ether (20 mL)
7. extractionThe water solution was extracted with diethyl ether (20 mL)
8. extractionthe combined diethyl ether extracts were extracted with acidic water (pH 5)
9. extractionextracted with ethyl acetate (3×50 mL)
10. washThe ethyl acetate solution was washed with pH 7 buffer solution (2×30 mL) and brine (30 mL)
11. dry with materialdried over Na2SO4
12. customAfter removing the solvent
13. addition0.6 N HCl methanol (10 mL) was added
14. concentrationthe solution was concentrated