huagongshe.com
HRID1065940

反应详情

EQUATION

反应方程式

HRID 1065940 的结构方程式

REACTANTS

反应物

3
结构：CO
HCID887

Methanol

CH4O

2 次

结构：O=P([O-])([O-])[O-]
HCID1061

Phosphate

O4P-3

结构：CC[C@H]1OC(=O)[C@H](C)[C@@H](O[C@H]2C[C@@](C)(OC)[C@@H](O)[C@H](C)O2)[C@H](C)[C@@H](O[C@@H]2O[C@H](C)C[C@H](N(C)C)[C@H]2O)[C@](C)(O)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@]1(C)O
HCID12560

Erythromycin

C37H67NO13

PRODUCTS

生成物

1
结构：CC[C@H]1OC(=O)[C@H](C)[C@@H](O[C@H]2C[C@@](C)(OC)[C@@H](O)[C@H](C)O2)[C@H](C)[C@@H](O[C@@H]2O[C@H](C)C[C@H](N(C)C)[C@H]2O)[C@](C)(OC)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@]1(C)O
HCID84029

Clarithromycin

C38H69NO13

PROCEDURE

实验过程

Each fraction was analyzed by thin layer chromatography (E. Merck Darmstadt. thin layer chromatography plate silica gel 60 silanized precoated, thickness 0.25 mm), developing in a mixture of methanol and a 0.1 M phosphate buffer solution (pH 7.0) (3:2). The fractions having Rf value 0.42 (c.f., erythromycin A, Rf value 0.46) were combined and most of the methanol was evaporated in vacuo. The residue was made alkaline with sodium carbonate and extracted with chloroform. The chloroform layer was washed with water, dried and concentrated in vacuo. The crystals obtained were recrystallized from a mixture of chloroform and diisopropyl ether (1:2), giving 700 mg of pure 6-O-methylerythromycin A (formula(I), R1 is hydrogen) in the form of colorless needles.

WORKUP

后处理

  1. customwas evaporated in vacuo
  2. extractionextracted with chloroform
  3. washThe chloroform layer was washed with water
  4. customdried
  5. concentrationconcentrated in vacuo
  6. customThe crystals obtained
  7. customwere recrystallized from a mixture of chloroform and diisopropyl ether (1:2)