反应详情

A solution of 8-chloro-7-(2-propen-1-yl)-3-propyl-3,7-dihydro-1H-purine-2,6-dione (200 mg, 0.74 mmol) in THF (4 ml) was treated with 3-[3-(phenylmethyl)-1,2,4-oxadiazol-5-yl]-1-propanol (195 mg, 0.89 mmol) and PPh3 (254 mg, 0.96 mmol). DBAD (223 mg, 0.96 mmol) was added in one portion and the mixture was left to stir at rt under nitrogen for 18 h. The mixture was partitioned between EtOAc and 2M HCl (aq). The organic layer was separated, washed with brine, dried (MgSO4) and concentrated. The crude product was purified by a silica SPE column using a 0-70% cyclohexane/EtOAc gradient. The product fractions were combined, concentrated and further purified by a silica SPE column using a 0-60% cyclohexane/EtOAc gradient. The product fractions were combined and concentrated then dissolved in anhydrous THF (4 ml). The solution was degassed by high vacuum then Pd(PPh3)4 (86 mg, 0.074 mmol) and morpholine (644 μl, 7.4 mmol) were added and the mixture left to stir at rt under nitrogen for 1 day. The mixture was partitioned between EtOAc and HCl (aq). The organic layer was separated, washed with brine, dried (MgSO4) and concentrated by high vacuum. The crude product was purified by an aminopropyl SPE using MeOH to load the compound onto the column and wash through the impurities then a 2-4% AcOH/MeOH gradient to elute the product. The product fractions were combined and concentrated by high vacuum to leave the title compound as a white solid (74 mg, 23%).

1. waitthe mixture was left
2. customThe mixture was partitioned between EtOAc and 2M HCl (aq)
3. customThe organic layer was separated
4. washwashed with brine
5. dry with materialdried (MgSO4)
6. concentrationconcentrated
7. customThe crude product was purified by a silica SPE column
8. concentrationconcentrated
9. customfurther purified by a silica SPE column
10. concentrationconcentrated
11. dissolutionthen dissolved in anhydrous THF (4 ml)
12. customThe solution was degassed by high vacuum
13. waitthe mixture left
14. stirringto stir at rt under nitrogen for 1 day
15. customThe mixture was partitioned between EtOAc and HCl (aq)
16. customThe organic layer was separated
17. washwashed with brine
18. dry with materialdried (MgSO4)
19. concentrationconcentrated by high vacuum
20. customThe crude product was purified by an aminopropyl SPE
21. washwash through the impurities
22. washa 2-4% AcOH/MeOH gradient to elute the product
23. concentrationconcentrated by high vacuum