反应详情

Tetrabutylammonium fluoride (1M in THF) (0.271 mL, 0.27 mmol) was added to (2S)-3-(2-(tert-butyldiphenylsilyloxy)ethoxy)-2-(1-(2-chlorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-yloxy)-N-(5-methylpyridin-2-yl)propanamide (Intermediate AG4) (96 mg, 0.14 mmol) in THF (5 mL) under nitrogen. The resulting mixture was stirred at room temperature for 1 hour. The reaction mixture was quenched with saturated NH4Cl (10 mL), extracted with Et2O (3×15 mL), the combined organic layers were back washed with water (10 mL), dried (MgSO4), and evaporated to afford crude product. The crude product was purified by flash silica chromatography, eluting with 0 to 10% MeOH in DCM to afford the product (50.0 mg, 79%). 1H NMR (400 MHz, CDCl3) δ2.28 (s, 3H), 3.32 (s, 1H), 3.70-3.81 (m, 4H), 4.13-4.26 (m, 2H), 6.08 (t, 1H), 7.43-7.55 (m, 4H), 7.59-7.63 (m, 1H), 8.05-8.10 (m, 2H), 8.44 (s, 1H), 8.59 (s, 1H), 9.11 (s, 1H); m/z (ESI+) (M+H)+=469.45; HPLC tR=1.86 min

1. customThe reaction mixture was quenched with saturated NH4Cl (10 mL)
2. extractionextracted with Et2O (3×15 mL)
3. washthe combined organic layers were back washed with water (10 mL)
4. dry with materialdried (MgSO4)
5. customevaporated
6. customto afford crude product
7. customThe crude product was purified by flash silica chromatography
8. washeluting with 0 to 10% MeOH in DCM