Hydrochloric Acid → (2S)-2-(3-(2-chlorophenyl)-3H-[1,2,3]triazolo[4,5-d]pyrimidin-7-yloxy)-3-(2-hydroxyethoxy)-N-(5-methylpyridin-2-yl)propanamide

HRID 1098041Notes

Reaction equation

HRID 1098041 reaction equation
Cl.[Si]([O:19][CH2:20][CH2:21][O:22][CH2:23][C@H:24]([O:35][C:36]1[C:37]2[N:44]=[N:43][N:42]([C:45]3[CH:50]=[CH:49][CH:48]=[CH:47][C:46]=3[Cl:51])[C:38]=2[N:39]=[CH:40][N:41]=1)[C:25]([NH:27][C:28]1[CH:33]=[CH:32][C:31]([CH3:34])=[CH:30][N:29]=1)=[O:26])(C(C)(C)C)(C1C=CC=CC=1)C1C=CC=CC=1>CO>[Cl:51][C:46]1[CH:47]=[CH:48][CH:49]=[CH:50][C:45]=1[N:42]1[C:38]2[N:39]=[CH:40][N:41]=[C:36]([O:35][C@@H:24]([CH2:23][O:22][CH2:21][CH2:20][OH:19])[C:25]([NH:27][C:28]3[CH:33]=[CH:32][C:31]([CH3:34])=[CH:30][N:29]=3)=[O:26])[C:37]=2[N:44]=[N:43]1

Participants

4

Reactant 2

HCID 313Hydrochloric AcidHCID 57504424(2S)-3-(2-(tert-butyldiphenylsilyloxy)ethoxy)-2-(3-(2-chlorophenyl)-3H-[1,2,3]triazolo[4,5-d]pyrimidin-7-yloxy)-N-(5-methylpyridin-2-yl)propanamide

Product 1

HCID 57504423(2S)-2-(3-(2-chlorophenyl)-3H-[1,2,3]triazolo[4,5-d]pyrimidin-7-yloxy)-3-(2-hydroxyethoxy)-N-(5-methylpyridin-2-yl)propanamideYield 30.3%

Reagents, catalysts & solvents 1

HCID 887MethanolSolvent

Conditions

Temperature
0 °C

Procedure

Hydrochloric acid (10%, 2 mL) was added to (2S)-3-(2-(tert-butyldiphenylsilyloxy)ethoxy)-2-(3-(2-chlorophenyl)-3H-[1,2,3]triazolo[4,5-d]pyrimidin-7-yloxy)-N-(5-methylpyridin-2-yl)propanamide (Intermediate AP3) (335 mg, 0.47 mmol) in methanol (30.0 mL) at 0° C. The resulting solution was stirred at 0° C. for 10 minutes and allowed to warm to room temperature and stirred for 1 hour. The reaction mixture was quenched with saturated NH4Cl (25 mL) and most of the MeOH was removed by evaporation. The aqueous residue was extracted with EtOAc (3×25 mL), the organic layer was dried (MgSO4), filtered and evaporated to afford crude product. The crude product was purified by preparative HPLC (Waters XBridge Prep C18 OBD column, 5μ silica, 50 mm diameter, 150 mm length), using decreasingly polar mixtures of water (containing 0.1% formic acid) and MeCN as eluents to afford the product (67.0 mg, 30.1%). 1H NMR (400 MHz, CDCl3) δ2.31 (s, 3H), 3.73-3.85 (m, 4H), 4.20-4.35 (m, 2H), 6.06 (t, 1H), 7.49-7.63 (m, 4H), 7.68 (d, 1H), 8.05 (s, 1H), 8.09-8.22 (m, 2H), 8.68 (s, 1H), OH not observed; m/z (ESI+) (M+H)+=470.41; HPLC tR=1.80 min

Workup

  1. temperatureto warm to room temperature
  2. stirringstirred for 1 hour
  3. customThe reaction mixture was quenched with saturated NH4Cl (25 mL) and most of the MeOH
  4. customwas removed by evaporation
  5. extractionThe aqueous residue was extracted with EtOAc (3×25 mL)
  6. dry with materialthe organic layer was dried (MgSO4)
  7. filtrationfiltered
  8. customevaporated
  9. customto afford crude product
  10. customThe crude product was purified by preparative HPLC (Waters XBridge Prep C18 OBD column, 5μ silica, 50 mm diameter, 150 mm length)

Sources & evidence

ORD record
ord-41ed15fe1aae44a8a66424a8d81b51f9
Source dataset
uspto-grants-2012_03
Patent number
US08143263B2

Reaction notes

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