反应详情

Pyridine (17 μL, 0.20 mmol) and 6-methoxy-m-toluenesulfonyl chloride (34 mg, 0.15 mmol) were added to a solution of tert-butyl 4-[(5-amino-1-benzofuran-7-yl)methyl]-3-methylpiperazine-1-carboxylate (45 mg crude material, 0.10 mmol; obtained in Step 2) in dry DCM:THF (2:1; 3 mL). The reaction mixture was stirred at room temperature for 2 h and the solvent was evaporated under reduced pressure followed by purification by flashtube (15% MeOH in DCM). The residue was dissolved in TFA:water (9:1; 4.5 mL) and the mixture was stirred at room temperature for 1 h to accomplish removal of the N-t-BOC group. The reaction mixture was diluted with DCM (10 mL) and saturated aqueous Na2CO3 was added to reach pH 8-9 (˜15 mL). The phases were separated using “phase separator” filters and the organic phase was concentrated. The crude product was purified by preparative HPLC (System B; 10-40% MeCN). Pure fractions were combined and concentrated to give the product as the free base, which was dissolved in MeOH and 1 M HCl in ether (200 μL, 0.2 mmol) was added. The solvent was evaporated to give the title compound (19 mg, 38%) as an off-white solid. HPLC 99%, RT=1.46 min (System A; 10-97% MeCN over 3 min), 100%, RT=1.29 min (System B; 10-97% MeCN over 3 min). 1H NMR (400 MHz, methanol-d4) δ ppm 1.72 (d, J=6.53 Hz, 3H) 2.20 (s, 3H) 3.39-3.62 (m, 5H) 3.67-3.74 (m, 1H) 3.87-3.96 (m, 1H) 3.98 (s, 3H) 4.60 (d, J=13.55 Hz, 1H) 4.93 (d, J=13.80 Hz, 1H) 6.87 (d, J=2.26 Hz, 1H) 7.04 (d, J=8.53 Hz, 1H) 7.28-7.37 (m, 2H) 7.52 (d, J=2.26 Hz, 1H) 7.54 (d, J=2.01 Hz, 1H) 7.85 (d, J=2.01 Hz, 1H). MS (ESI+) for C22H27N3O4S m/z 430 (M+H)+.

1. customthe solvent was evaporated under reduced pressure
2. customfollowed by purification by flashtube (15% MeOH in DCM)
3. dissolutionThe residue was dissolved in TFA:water (9:1; 4.5 mL)
4. stirringthe mixture was stirred at room temperature for 1 h
5. customremoval of the N-t-BOC group
6. additionThe reaction mixture was diluted with DCM (10 mL) and saturated aqueous Na2CO3
7. additionwas added
8. customThe phases were separated
9. concentration” filters and the organic phase was concentrated
10. customThe crude product was purified by preparative HPLC (System B; 10-40% MeCN)
11. concentrationconcentrated
12. customto give the product as the free base, which
13. customThe solvent was evaporated