反应详情

8-Cyclohexyl-2-(3-imidazol-1-yl-propylamino)-5-oxo-5,8-dihydro-pyrido[2,3-d]pyrimidine-6-carboxylic acid ethyl ester (4.5 mg, 0.011 mmol) was added to 1.0 mL of 7 N ammonia in methanol. The mixture was stirred in a sealed tube at room temperature overnight. The solvent was evaporated to leave a white solid of 8-cyclohexyl-2-(3-imidazol-1-yl-propylamino)-5-oxo-5,8-dihydro-pyrido[2,3-d]pyrimidine-6-carboxylic acid amide (4.0 mg, 95%). 1H NMR (400 MHz, CDCl3/CD3OD (20:1 v/v)) δ (ppm): 9.18 (s), 8.74 (s), 7.15 (br, 1H), 7.02 (br, 1H), 6.92 (br, 1H), 5.02 (m, 1H), 4.06 (t, J=6.6 Hz, 2H), 3.43 (br, 2H), 2.17 (m, 2H), 2.00-1.30 (br, 10H). Mass Spectrum (LCMS, ESI pos.) Calcd. For C20H25N7O2: 396.21 (M+H). Found: 396.2.

1. customThe solvent was evaporated