反应详情

The t-butyl (2-(5-bromo-1-((5-chlorothiazol-2-yl)carbamoyl)spiro[indoline-3,3′-pyrrolidin]-1′-yl)-2-oxoethyl)carbamate (18 mg, 0.032 mmol) obtained in Example 19 was dissolved in ethyl acetate (0.5 mL). Thereafter, a 4 N solution of HCl/ethyl acetate (200 μL, 0.800 mmol) was added to the above obtained solution at room temperature, and the obtained mixture was then stirred for 7 hours. Thereafter, the reaction solution was neutralized, was then filtered, and was then concentrated in vacuo. The obtained residue was purified by silica gel column chromatography (chloroform:ammonia-methanol=10:1) to obtain the captioned compound in the form of a light yellow solid.

1. filtrationwas then filtered
2. concentrationwas then concentrated in vacuo
3. customThe obtained residue was purified by silica gel column chromatography (chloroform:ammonia-methanol=10:1)
4. customto obtain the captioned compound in the form of a light yellow solid