反应详情

Ceftiofur sodium (82 mg)(The Upjohn Co., Kalamazoo, Mich.) was dissolved in 500 μL PBS-A, and 10 mg sulfosuccinimidyl 4-(p-maleimidophenyl)-butyrate (s-SMPB)(Pierce, Rockford, Ill.) in 20 μL DMF was added. The resulting mixture was stirred for 10-15 min, added dropwise to 30 mg BSA-SH (1.5 mL of a 20 mg/mL stock solution), stirred overnight at ambient temperature, and then dialyzed exhaustively against PBS-A. Similarly, 52 mg ceftiofur sodium in PBS-A and 5 mg s-SMPB in 20 μL N,N-dimethylformamide (DMF) were added dropwise to 20 mg of OVA-SH (1 mL of a 20 mg/mL stock solution), and the mixture was stirred overnight at ambient temperature and dialyzed exhaustively against PBS-B (0.05M sodium phosphate, 0.075M sodium chloride in deionized water, pH 7.2). These conjugates were abbreviated as BSA-S-SMPB-Cef and OVA-S-SMPB-Cef, respectively.

1. additionwas added
2. additionadded dropwise to 30 mg BSA-SH (1.5 mL of a 20 mg/mL stock solution),
3. stirringstirred overnight at ambient temperature
4. customdialyzed exhaustively against PBS-A
5. additionSimilarly, 52 mg ceftiofur sodium in PBS-A and 5 mg s-SMPB in 20 μL N,N-dimethylformamide (DMF) were added dropwise to 20 mg of OVA-SH (1 mL of a 20 mg/mL stock solution), and the mixture
6. stirringwas stirred overnight at ambient temperature
7. customdialyzed exhaustively against PBS-B (0.05M sodium phosphate, 0.075M sodium chloride in deionized water, pH 7.2)