反应详情

These compounds were prepared from the corresponding compounds I-III by removal of protecting groups with HBr in acetic acid. The benzyloxycarbonyl and t-butyloxycarbonyl groups were simultaneously removed by treatment with 30% Hbr in acetic acid containing anisol (10%) for 15 minutes at room temperature. Yields were about 90%. The molar ratios of the constituent amino acid residues and electrophoretic migrations relative to lysine were for compounds IV: Ala/Lys=1.05 (calc. 1.00), RLys =0.55, for compound V: Phe/Lys=1.06 (calc. 1.00), RLys =0.46, for compound VI: Ala/Lys=1.98 (calc. 2.00); RLys =0.51. Incubation of each of the compounds IV, V and VI with leucine aminopeptidase (E.C. 3.4.11.1) produced quantitatively ε(2-aminobenzoyl)-L-lysisne, (RLys =0.26) and the other expected product as judged by high voltage paper electrophoresis using authentic markers.

1. customThese compounds were prepared from the corresponding compounds
2. customby removal of protecting groups with HBr in acetic acid
3. customThe benzyloxycarbonyl and t-butyloxycarbonyl groups were simultaneously removed by treatment with 30% Hbr in acetic acid
4. additioncontaining anisol (10%) for 15 minutes at room temperature
5. customYields