反应详情

Pd-C (5%) (500 mg) was added to a solution of 2,6-diazido-9-(2,3,5-tri-O-benzyl-β-D-arabinofuranosyl)purine (IV, 4.8 g, 8.0 mmol) in EtOH (500 ml), and the resulting mixture was hydrogenated at atmospheric pressure for 6 hr. The H2 atmosphere was changed after 30 min. and after 1 hr. The catalyst was removed by filtration and was washed with CHCl3 to dissolve precipitated product. The combined filtrate and washings were evaporated to dryness in vacuo, and the resulting residue was dissolved in boiling EtOH (80 ml). The crystals that formed on cooling the EtOH solution were collected by filtration, washed with EtOH, and dried in vacuo; yield 3.3 g (75%), mp 161°-162°, TLC (19:1 CHCl3 --MeOH). The analytically pure produce was obtained from a previous preparation: yield 69%; mp 162°; [α]D25 +44.4±0.7° (c, 1.06, CHCl3); λmax ]Mμ (ε× 10-3)], pH 1--254 (10.9), 292 (9.0); EtOH--256 (10.8), 283 (9.0); pH 7, 13--256 (9.0), 279 (9.1); vmax (cm-1)3460, 3350, 3310, 3140 (NH, CH), 2940, 2915 2890, 2865 (CH), 1660 (NH), 1590 (C=C, C=N), 1085, 1045, 1015 (COC), 730, 690 (phenyl).

1. waitThe H2 atmosphere was changed after 30 min. and after 1 hr
2. customThe catalyst was removed by filtration
3. washwas washed with CHCl3
4. dissolutionto dissolve
5. customprecipitated product
6. customThe combined filtrate and washings were evaporated to dryness in vacuo
7. dissolutionthe resulting residue was dissolved
8. customThe crystals that formed
9. filtrationwere collected by filtration
10. washwashed with EtOH
11. customdried in vacuo
12. customThe analytically pure produce
13. customwas obtained from a previous preparation