反应详情

The title compound was prepared from methyl 4-(benzyloxy)-5-{[(2-hydroxy-2-pyridin-2-ylethyl)(methyl)amino]carbonyl}-1H-pyrazole-3-carboxylate using a procedure similar to that described in Example 43, Steps 2-4, except that 4-fluoro-2-[(methylamino)carbonyl]benzylamine hydrochloride was used in place of 4-fluorobenzylamine. Purification was achieved by removing the solvent in vacuo, and purifying the resulting residue by reverse phase chromatography on a C-18 column using a gradient elution of 95-5% H2O (0.1% TFA)/CH3CN (0.1% TFA). Collection and concentration of the appropriate fractions afforded the product. ES MS (M+1)=543.

1. customPurification
2. customby removing the solvent in vacuo
3. custompurifying the resulting residue by reverse phase chromatography on a C-18 column
4. washa gradient elution of 95-5% H2O (0.1% TFA)/CH3CN (0.1% TFA)
5. customCollection and concentration of the appropriate fractions
6. customafforded the product