反应详情

Benzhydryl 7-[(Z)-2-(2-tritylaminothiazol-4-yl)-2-trityloxyiminoacetamido]-3-[(Z)-2-(4-methyl-1,2,3-thiadiazol-5-yl)vinyl]-3-cephem-4-carboxylate (1.38 g, 1.2 mmol) was stirred at room temperature for 1 hour by addition of formic acid (14 ml). Concentrated hydrochloric acid (0.11 ml) was added to the reaction solution and the mixture was stirred at room temperature for 1 hour. The reaction solution was concentrated under reduced pressure and the residue was cleansed with ether thereby a yellow powder product was obtained. This one was dissolved in aqueous sodium bicarbonate solution and purified by HP-20 column chromatography thereby sodium 7-[(Z)-2-(2-aminothiazol-4-yl)-2-hydroxyiminoacetamido]-3-[(Z)-2-(4-methyl-1,2,3-thiadiazol-5-yl)vinyl]-3-cephem-4-carboxylate (79 mg) was obtained.

1. concentrationThe reaction solution was concentrated under reduced pressure
2. customa yellow powder product was obtained
3. custompurified by HP-20 column chromatography