反应详情

EDCI (356 mg) and HOBT (306 mg) were added to a solution of 5-chloro-6-[(1-methylethyl)oxy]-3-pyridinecarboxylic acid (200 mg) in THF (5 mL) at RT. The resulting solution was stirred for 30 mins. Ethyl 3-{6-[(Z)-(hydroxyamino)(imino)methyl]-1H-indol-3-yl}propanoate (D107) (383 mg) in THF (5 mL) was added and the reaction mixture was stirred at RT for 2 hours. TBAF (970 mg) was then added. The reaction vessel was sealed and heated in Biotage Initiator using initial normal to 120° C. for 90 mins. After cooling, the reaction was quenched with water, and extracted with EtOAc for 3 times. The combined organic solution was washed with brine, dried over anhydrous sodium sulfate. The dried solution was concentrated. The residue was purified by column chromatography (20% EtOAc in hexane) to afford ethyl 3-[6-(5-{5-chloro-6-[(1-methylethyl)oxy]-3-pyridinyl}-1,2,4-oxadiazol-3-yl)-1H-indol-3-yl]propanoate (D108) (310 mg) as a pale yellow solid. δH (CDCl3, 400 MHz): 1.25 (3H, t), 1.46 (6H, d), 2.74 (2H, t), 3.15 (2H, t), 4.15 (2H, q), 5.51 (1H, m), 7.17 (1H, d), 7.72 (1H, d), 7.93 (1H, dd), 8.20 (2H, d), 8.42 (1H, d), 8.89 (1H, d). MS (ES): C23H23ClN4O4 requires 454; found 455.2 (M+H+).

1. stirringthe reaction mixture was stirred at RT for 2 hours
2. customThe reaction vessel was sealed
3. temperatureheated in Biotage Initiator
4. customnormal to 120° C.
5. customfor 90 mins
6. temperatureAfter cooling
7. customthe reaction was quenched with water
8. extractionextracted with EtOAc for 3 times
9. washThe combined organic solution was washed with brine
10. dry with materialdried over anhydrous sodium sulfate
11. concentrationThe dried solution was concentrated
12. customThe residue was purified by column chromatography (20% EtOAc in hexane)