反应详情

温度

70 °C

N-(6-(2,5-Difluorophenyl)-2,3-dimethylpyridin-4-yl)-1-(4-methoxybenzyl)-2H-pyrazolo[4,3-b]pyridin-7-amine and N-(6-(2,5-difluorophenyl)-2,3-dimethylpyridin-4-yl)-2-(4-methoxybenzyl)-2H-pyrazolo[4,3-b]pyridin-7-amine was combined in TFA (5 mL) and heated at 70° C. for 12 hours. The reaction mixture was evaporated to give a residue which was purified via preparative HPLC using a gradient of 5-30% acetonitrile (containing 0.035% TFA) in water (containing 0.05% TFA) using a Sunfire Prep 5 μm C18, 75×30 mm column to give the title compound as a TFA salt. 1H NMR (400 MHz, METHANOL-d4) δ ppm 1.93 (s, 1H) 2.24 (s, 1H) 2.42 (s, 3H) 2.75 (s, 3H) 6.96 (br. s., 1H) 7.23-7.33 (m, 2H) 7.60 (s, 1H) 7.61-7.68 (m, 1H) 8.44 (s, 2H). MS [M+H] found 352.1

1. customThe reaction mixture was evaporated
2. customto give a residue which
3. customwas purified via preparative HPLC