反应详情

30 g (1.0 eq) Boc-D-Glu(OBzl)-NH2 was dissolved in 100 mL trifluoroacetic acid-dichloromethane (v/v=1:1), and the solution was stirred for 1 hour at r.t to remove Boc group. After the completion of the reaction, the TFA was removed under vacuum; the residue was repeatedly grinded and washed in anhydrous ether, and evaporated to dryness, and re-dissolved in tetrahydrofuran. The pH of the solution was adjusted to 7˜8 with N-methyl morpholine (NMM) in ice bath. The Boc-Ala-OSu solution was sparingly added to the solution in a few portions. The mixture was stirred for 5 hours in ice bath, and continued for 24 hours at r.t. After the completion of the reaction, the mixture was evaporated to dryness. The residue was dissolved in proper amount AcOEt and successively washed with diluted hydrochloric acid, saturated sodium bicarbonate aqueous solution and water. The AcOEt layer was separated, and dried with MgSO4 overnight. The mixture was filtered and the filtrate was evaporated to dryness. The residue was recrystallized from methanol and water, the crystal was washed with a big amount of ether to obtained 29.4 g target compound. Yield: 81%, m.p.=134˜135° C.

1. customto remove Boc group
2. customAfter the completion of the reaction
3. customthe TFA was removed under vacuum
4. customthe residue was repeatedly grinded
5. washwashed in anhydrous ether
6. customevaporated to dryness
7. dissolutionre-dissolved in tetrahydrofuran
8. additionThe Boc-Ala-OSu solution was sparingly added to the solution in a few portions
9. stirringThe mixture was stirred for 5 hours in ice bath
10. waitcontinued for 24 hours at r.t
11. customAfter the completion of the reaction
12. customthe mixture was evaporated to dryness
13. dissolutionThe residue was dissolved in proper amount AcOEt
14. washsuccessively washed with diluted hydrochloric acid, saturated sodium bicarbonate aqueous solution and water
15. customThe AcOEt layer was separated
16. dry with materialdried with MgSO4 overnight
17. filtrationThe mixture was filtered
18. customthe filtrate was evaporated to dryness
19. customThe residue was recrystallized from methanol and water
20. washthe crystal was washed with a big amount of ether
21. customto obtained 29.4 g target compound