反应详情

To a solution of (2S,4R)-2-(3-bromo-5-methoxycarbonyl-phenylcarbamoyl)-4-fluoro-pyrrolidine-1-carboxylic acid tert-butyl ester (480 mg, 1.08 mmol) in MeOH (10 mL), cooled to 0° C. was added LiOH.H2O (271 mg, 6.47 mmol) and the resulting solution was allowed to reach RT and stirred for 5 h. MeOH was concentrated and the residue was diluted in CH2Cl2, HCl 1 M was added (pH=1) and the layers were separated. The aqueous layer was back extracted twice with CH2Cl2 and the combined organic layers were dried over Na2SO4, filtered and concentrated to give the desired material which was used without further purification in the next step. MS (LC-MS): 453.0/455.0 [M+Na]+, 375.1/377.1 [MH−tBu]+, 331.1/333.1 [MH−Boc]+, 429.0/431.0 [M−H]−; tR (HPLC conditions a): 3.27 min.

1. customto reach RT
2. concentrationMeOH was concentrated
3. additionthe residue was diluted in CH2Cl2
4. additionHCl 1 M was added (pH=1)
5. customthe layers were separated
6. extractionThe aqueous layer was back extracted twice with CH2Cl2
7. dry with materialthe combined organic layers were dried over Na2SO4
8. filtrationfiltered
9. concentrationconcentrated