反应详情

3-(1.6-dihydro-6-oxo-9H-purin-9-yl)propionic acid, ethyl ester (250 mg. 1.06 mol) (AIT-0027) was placed into a 10 ml round bottom flask equipped with a magnetic stirring bar, reflux condenser and CaCl2 drying tube. Then 3 ml acetonitrile and 280 mg (2.18 mmol) 2-(aminomethyl)-1-ethyl-pyrrolidine were added and the solution was heated to reflux. Not all of the ester dissolved at reflux, but as the reaction proceeded, the solution became homogeneous. Reflux was continued for 17 hours, at which time the solvent had completely evaporated. The residue was treated with acetonitrile and allowed to cool. A white precipitate had formed. Ether was added and the solution was stirred to wash the precipitate. The solution was filtered by Buchner vacuum filtration and the precipitate was washed with ether. Upon drying, 300 mg (0.94 mmol) of 3-(1,6-dihydro-6-oxo-9H-purin-9-yl)-N-[(1-ethyl-2-pyrrolidinyl)methyl]propanamide (AIT-0029) was obtained as an off-white solid.

1. customequipped with a magnetic stirring bar
2. temperaturereflux condenser and CaCl2 drying tube
3. temperaturethe solution was heated to reflux
4. temperatureat reflux
5. temperatureReflux
6. customthe solvent had completely evaporated
7. additionThe residue was treated with acetonitrile
8. temperatureto cool
9. customA white precipitate had formed
10. washto wash the precipitate
11. filtrationThe solution was filtered by Buchner vacuum filtration
12. washthe precipitate was washed with ether
13. customUpon drying