反应详情

To a solution of 17-ethyl-1,14-dihydroxy-12-[2'-(3",4"-dihydroxycyclohexyl)-1'-methylvinyl]-23,25-dimethoxy-13,19,21,27-tetramethyl-11,28-dioxa-4-azatricyclo[22.3.1.04,9 ]octacos-18-ene-2,3,10,16-tetraone (150 mg in 3 ml 33% methylene chloride in cyclohexane), sec-butenyl trichloroacetimidate (62 μl neat) was added and the reagents allows to mix for 5 minutes. Trifluoromethanesulfonic acid (2 μl neat) was added slowly via syringe and the mixture stirred at room temperature. After 15 hours the reaction was quenched by the addition of saturated sodium bicarbonate and extracted with ethyl acetate (3×8 ml). The combined organic were washed with brine and dried over magnesium sulfate. Purification of the concentrate by preparative TLC on silica gel (ethyl acetate:hexane (1:1)+1% methanol) gave the title compounds (11 mg 4"-ether; 3"-ether).

1. additionto mix for 5 minutes
2. customAfter 15 hours the reaction was quenched by the addition of saturated sodium bicarbonate
3. extractionextracted with ethyl acetate (3×8 ml)
4. washThe combined organic were washed with brine
5. dry with materialdried over magnesium sulfate
6. customPurification of the
7. concentrationconcentrate by preparative TLC on silica gel (ethyl acetate:hexane (1:1)+1% methanol)