反应详情

To a solution of 4-{2-[2-(4-chloro-phenyl)-4,5,6,7-tetrahydro-2H-indazol-3-yl]-2-cyclohexyl-2-hydroxy-acetylamino}-3-fluoro-benzoic acid ethyl ester (6 mg, 11 μmol) in THF/MeOH 1/1 (0.2 ml) was a added a 1 N aqueous lithium hydroxide solution (60 μl, 60 μmol) at ambient temperature under an argon atmosphere. The reaction mixture was stirred for 12 h at ambient temperature and poured onto ice water/1 N aqueous NaOH solution 1/1. The layers were separated, the aqueous layer was acidified with ice cold 1 N aqueous HCl solution and extracted two times with iPrOAc. The combined extracts were washed with ice water/brine 1/1 and dried over Na2SO4. After filtration the solvent was removed under reduced pressure to give the title compound (7 mg; quant.) as yellow solid. MS: m/e=526.4 [M+H+].

1. additionpoured onto ice water/1 N aqueous NaOH solution 1/1
2. customThe layers were separated
3. extractionextracted two times with iPrOAc
4. washThe combined extracts were washed with ice water/brine 1/1
5. dry with materialdried over Na2SO4
6. filtrationAfter filtration the solvent
7. customwas removed under reduced pressure