反应详情

(6R,7R)-7-(2-Amino-4-thiazoleglyoxylamido)-3-[[[2-(hydroxycarbamoyl)-5-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (65 mg) (0.11 mmol) are dissolved in 4 ml of absolute dimethylacetamide. 24 mg (0.14 mmol) of 2-[(aminooxy)methyl]-5-hydroxy-3-methyl-4(3H)-pyrimidinone and 14 mg (0.14 mmol) of methanesulphonic acid are added. After stirring at room temperature for 20 hours the dimethylacetamide is evaporated in a high vacuum and the residue is taken up in water and stirred for 15 minutes in order to precipitate the product completely. The product is filtered off, washed with water and diethyl ether and dried in a high vacuum at room temperature. There are obtained 48 mg of (6R,7R)-7-[(Z)-2-(2-amino-4-thiazolyl)-2-[[(1,6-dihydro-5-hydroxy-1-methyl-6-oxo-2-pyrimidinyl)methoxy]imino]acetamido]-3-[ [[2-(hydroxycarbamoyl)-5-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid as a beige powder.

1. customis evaporated in a high vacuum
2. customto precipitate the product completely
3. filtrationThe product is filtered off
4. washwashed with water and diethyl ether
5. customdried in a high vacuum at room temperature