反应详情

(6R,7R)-7-(2-Amino-4-thiazoleglyoxylamido)-3-[[[5-[2-(hydroxycarbamoyl)ethyl]-2-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid (41 mg) (0.66 mmol) and 29 mg of 1-[2-(aminooxy)-2-methylpropionyl]-2-(3,4-dihydroxybenzoyl)hydrazine hydrochloride are dissolved in 1 ml of absolute dimethylacetamide. After stirring at room temperature for 20 hours the solvent is removed at room temperature in a high vacuum. The residue is crystallized from ethanol/diethyl ether. The product is filtered off, washed with diethyl ether, water and again with diethyl ether. After drying in a high vacuum at room temperature there are obtained 45 mg of (6R,7R)-7-[(Z)-2-(2-amino-4-thiazolyl)-2-[[1-[3-(3,4-dihydroxybenzoyl)carbazoyl]-1-methylethoxy]imino]acetamido]-3-[[[5-[2-(hydroxycarbamoyl)ethyl]-2-methyl-s-triazolo[1,5-a]pyrimidin-7-yl]thio]methyl]- 8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid as a beige powder.

1. customis removed at room temperature in a high vacuum
2. customThe residue is crystallized from ethanol/diethyl ether
3. filtrationThe product is filtered off
4. washwashed with diethyl ether, water and again with diethyl ether
5. customAfter drying in a high vacuum at room temperature