反应详情

A solution of 0.60 g of tert.butyl 1,2,3,4-tetrahydropyrido[3,4-b]indole-1-carboxamide and 0.66 g of 3(S)-(benzyloxyformamido)-1,2(S)-epoxy-4-phenylbutane in 20 ml of methanolwas heated at reflux under argon for 16 hours and then evaporated to give aclear oil. The two diastereomeric products were separated by flash chromatography on silica gel using n-hexane/ethyl acetate (3:1) for the elution. There were obtained 268 mg of isomer A of 2-[3(S)-(benzyloxyformamido)-2(R)-hydroxy-4-phenylbutyl]-N-tert.butyl-1,2,3,4-tetrahydropyrido[3,4-b]indole-1-carboxamide, MS: m/e 569 [M+H]+ and 65 mg of isomer B of the same compound; MS: m/e 569 [M+H]+.

1. temperatureat reflux under argon for 16 hours
2. customevaporated
3. customto give aclear oil
4. customThe two diastereomeric products were separated by flash chromatography on silica gel
5. washfor the elution