反应详情

23.0 g (40 mmoles) of the protected tripeptide ester obtained in Step 2 above are dissolved in 40 ml of methanol, and the mixture is hydrogenated in the presence of palladium-on-carbon catalyst. At the end of the reaction the catalyst is filtered off, washed with methanol, the filtrate and the wash are combined and evaporated under reduced pressure. The residue is triturated with diethyl ether, the solid is filtered off, washed with diethyl ether and dried in a vacuum desiccator. 17.45 g (89.8%) of tert.-butoxycarbonyl-L-tyrosyl-D-phenylalanyl-glycine are obtained; m.p.: 105°-110° C., Rf13 =0.2-0.3.

1. customAt the end of the reaction the catalyst
2. filtrationis filtered off
3. washwashed with methanol
4. customevaporated under reduced pressure
5. customThe residue is triturated with diethyl ether
6. filtrationthe solid is filtered off
7. washwashed with diethyl ether
8. customdried in a vacuum desiccator