反应详情

温度

90 °C

To a solution of N-{3-[(2-amino[1,3]thiazolo[5,4-d]pyrimidin-5-yl)amino]-4-methylphenyl}-3-(1-cyano-1-methylethyl)benzamide (82 mg, 185 μmol) produced in Example D25(iii) in pyridine (3 mL) were added 5-(1-methylethyl)-1H-pyrazole-3-carboxylic acid (57 mg, 370 μmol) and O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (211 mg, 555 μmol), and the mixture was stirred at 90° C. for 21 hr. To the reaction mixture was added saturated aqueous sodium hydrogen carbonate solution (15 mL), and the mixture was extracted with ethyl acetate (20 mL, 5 mL). The combined organic layer was washed with saturated brine (5 mL), and dried over anhydrous sodium sulfate. The insoluble material was filtered off, and the filtrate was concentrated under reduced pressure. The obtained residue was purified by basic silica gel column chromatography (ethyl acetate/methanol=100/0→92/8), and the fractions containing the object product were concentrated under reduced pressure. The obtained residue was recrystallized from N,N-dimethylformamide and diethyl ether to give the title compound (34 mg, 32%) as pale-yellow crystals.

1. extractionthe mixture was extracted with ethyl acetate (20 mL, 5 mL)
2. washThe combined organic layer was washed with saturated brine (5 mL)
3. dry with materialdried over anhydrous sodium sulfate
4. filtrationThe insoluble material was filtered off
5. concentrationthe filtrate was concentrated under reduced pressure
6. customThe obtained residue was purified by basic silica gel column chromatography (ethyl acetate/methanol=100/0→92/8)
7. additionthe fractions containing the object product
8. concentrationwere concentrated under reduced pressure
9. customThe obtained residue was recrystallized from N,N-dimethylformamide and diethyl ether