反应详情

To a mixture of (4-{3-[6-(2-tert-Butoxycarbonylamino-1-phenyl-ethylamino)-pyridin-2-yl]-1-trityl-1H-pyrazolo[3,4-d]pyrimidin-6-ylamino}-cyclohexyl)-carbamic acid tert-butyl ester (103 mg, 0.116 mmol) in C2H5OH (8 mL) was added conc. HCl (8 mL) at room temperature. The mixture was stirred at room temperature for 15 hour. The solvent was removed under reduced pressure. The residue was washed with dichloromethane (10 mL) to get the crude compound which was purified by prep-HPLC to give N-{3-[6-(2-amino-1-phenyl-ethylamino)-pyridin-2-yl]-1H-pyrazolo[3,4-d]pyrimidin-6-yl}-cyclohexane-1,4-diamine; hydrochloride as a yellow solid. (Yield 14 mg).

1. customThe solvent was removed under reduced pressure
2. washThe residue was washed with dichloromethane (10 mL)
3. customto get the crude compound which
4. customwas purified by prep-HPLC