反应详情

To a solution of 2-formamido-4-carboxymethylthiazole (2.0 g) in tetrahydrofuran (20 ml) was added N-chlorosuccinimide (1.58 g) and stirred at room temperature overnight. The reaction mixture was added N-chlorosuccinimide (0.5 g) and stirred at the same temperature overnight. The reaction mixture was evaporated under reduced pressure and purified by column chromatography on silica gel (SiO2 =200 ml, chloroform:methanol:acetic acid=20:1:0.1) to give two fractions. The fraction 1 (upper spot by TLC) was purified by column chromatography on silica gel (SiO2 =200 ml, methanol:chloroform=2:8), then the elution was evaporated under reduced pressure. The residue was dissolved in a mixture of water and ethyl acetate, adjusted to pH 8.7 with saturated sodium hydrogencarbonate, and washed with ethyl acetate (×2). The aqueous layer was adjusted to pH 3.0 with 1N-hydrochloric acid, extracted with ethyl acetate (×2), dried over magnesium sulfate, and evaporated under reduced pressure to give white solid which was precipitated from ethyl acetate and N-hexane to give 5-chloro-2-formamido-4-carboxymethylthiazole (694 mg, 29.3%) as a white powder.

1. stirringstirred at the same temperature overnight
2. customThe reaction mixture was evaporated under reduced pressure
3. custompurified by column chromatography on silica gel (SiO2 =200 ml, chloroform:methanol:acetic acid=20:1:0.1)
4. customto give two fractions
5. customThe fraction 1 (upper spot by TLC) was purified by column chromatography on silica gel (SiO2 =200 ml, methanol:chloroform=2:8)
6. washthe elution
7. customwas evaporated under reduced pressure
8. dissolutionThe residue was dissolved in a mixture of water and ethyl acetate
9. washwashed with ethyl acetate (×2)
10. extractionextracted with ethyl acetate (×2)
11. dry with materialdried over magnesium sulfate
12. customevaporated under reduced pressure
13. customto give white solid which
14. customwas precipitated from ethyl acetate and N-hexane