反应详情

4-[2-(tert-Butoxycarbonylamino-methyl)-morpholin-4-yl]-9H-pyrimido[4,5-b]indole-7-carboxylic acid (6 mg, 0.014 mmol) was stirred in DMF (0.25 mL) at 0° C. Carbonydiimidazole (2.5 mg, 0.016 mmol) was added and the reaction allowed to proceed to room temperature for 30 minutes. Ammonia (28% aqueous, 0.04 mL) was added. The reaction mixture was concentrated to dryness and then the reaction was repeated to afford a complete conversion. The reaction mixture was concentrated to give the title compound as a yellow solid (5 mg, 0.012 mmol), which was deprotected without further purification. LC-MS (1) Rt 1.48 min; m/z 427 (ES+).

1. additionCarbonydiimidazole (2.5 mg, 0.016 mmol) was added
2. concentrationThe reaction mixture was concentrated to dryness
3. customto afford a complete conversion
4. concentrationThe reaction mixture was concentrated