反应详情

3-chloro-5-[(4-methyl-2-oxo-1-{2-[1-(tetrahydro-2H-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridin-3-yl]ethyl}-1,2-dihydropyridin-3-yl)oxy]benzonitrile (11.7 mg, 0.023 mmol) was dissolved in TFA (2 mL) and allowed to stand at room temperature for 1 hour. After this time, MeOH (1 mL) was added and the solvent evaporated in vacuo. The residue was purified on Gilson LC using a Luna reverse phase column (10μ, C18, 250×21.2 mm) and eluting with a gradient of 5-95% ACN/water (0.5% TFA). The desired fractions were combined and the solvent evaporated in vacuo to give the title compound. LRMS (M+1): 405.9.

1. customthe solvent evaporated in vacuo
2. customThe residue was purified on Gilson LC
3. washeluting with a gradient of 5-95% ACN/water (0.5% TFA)
4. customthe solvent evaporated in vacuo