反应详情

A solution of 4-(1H-pyrrol-3-yl)-7-{[2-(trimethylsilyl)ethoxy]methyl}-7H-pyrrolo[2,3-d]pyrimidine (0.100 g, 0.318 mmol) and [1-(ethylsulfonyl)azetidin-3-ylidene]acetonitrile (0.118 g, 0.636 mmol, prepared as in Example 68) in acetonitrile (1 mL) was treated with 1,8-diazabicyclo[5.4.0]undec-7-ene (24 μL, 0.16 mmol). The mixture was stirred for 4 hours. The solvent was evaporated. The residue was partitioned between ethyl acetate and saturated sodium bicarbonate solution, then extracted with two further portions of ethyl acetate. The combined extracts were dried over sodium sulfate, decanted and concentrated. The crude product was stirred with 25% TFA/DCM (8 mL) overnight. The solvents were evaporated. The product was then stirred with ethylenediamine (0.3 mL) in methanol (5 mL). Preparative HPLC-MS (eluting with a gradient of methanol and water containing 0.15% NH4OH) was used to purify the product. 1H NMR (300 MHz, CDCl3): δ 8.59 (s, 1H), 7.54 (dd, 1H), 7.27 (d, 1H), 6.95 (dd, 1H), 6.82 (dd, 1H), 6.74 (d, 1H), 4.49 (d, 2H), 4.15 (d, 2H), 3.24 (s, 2H), 3.02 (q, 2H), 1.33 (t, 3H); LCMS (M+H)+: 371.1.

1. customThe solvent was evaporated
2. customThe residue was partitioned between ethyl acetate and saturated sodium bicarbonate solution
3. extractionextracted with two further portions of ethyl acetate
4. dry with materialThe combined extracts were dried over sodium sulfate
5. customdecanted
6. concentrationconcentrated
7. stirringThe crude product was stirred with 25% TFA/DCM (8 mL) overnight
8. customThe solvents were evaporated
9. stirringThe product was then stirred with ethylenediamine (0.3 mL) in methanol (5 mL)
10. washPreparative HPLC-MS (eluting with a gradient of methanol and water containing 0.15% NH4OH)
11. customto purify the product