反应详情

To a solution of tert-butyl 4-(2-((6-(2-cyanophenyl)-2-(3-fluorophenethylamino)nicotinamido)methyl)pyrrolidin-1-yl)-4-oxobutylcarbamate (71 mg, 0.11 mmol) in MeOH (5.0 mL) was added HCl (1.0 mL, 4 mmol, 4 M in dioxane). The reaction mixture was stirred at r.t. for 2 h. LC/MS indicated the reaction was complete. The reaction mixture was concentrated under reduced pressure and the crude was purified on RP-HPLC using a mixture of acetonitrile (0.1% TFA) and water (0.1% TFA) to give N-((1-(4-aminobutanoyl)pyrrolidin-2-yl)methyl)-6-(2-cyanophenyl)-2-(3-fluorophenethylamino)nicotinamide as a pale yellow solid (57.1 mg, 68%). LRMS (M+H+) m/z 529.2.

1. concentrationThe reaction mixture was concentrated under reduced pressure
2. customthe crude was purified on RP-HPLC
3. additiona mixture of acetonitrile (0.1% TFA) and water (0.1% TFA)