反应详情

To a solution of 17-ethyl-20-fluoro-1,14-dihydroxy-12-[2'-(4"-hydroxy-3"-methoxycyclohexyl)-1'-methylvinyl]-23,25-dimethoxy-13,19,21,27-tetramethyl-11,28-dioxa-4-azatricyclo[22.3.1.04,9 ]octacos-18-ene-2,3,10,16-tetraone (50 mg in 1.5 ml 33% methylene chloride in cyclohexane), 2-butynyl trichloroacetimidate (20 μl neat) is added and the reagents are allowed to mix for 5 minutes. Trifluoromethanesulfonic acid (2 μl neat) is added slowly via syringe acid (2 μl neat) is added and the mixture is stirred at room temperature. After 16 hours the reaction is quenched by the addition of saturated sodium bicarbonate and extracted with ethyl acetate (3×5 ml). The combined organic are washed with brine and dried over magnesium sulfate. Purification of the concentrate by preparative TLC on silica gel gives the title compound.

1. additionto mix for 5 minutes
2. additionis added
3. customAfter 16 hours the reaction is quenched by the addition of saturated sodium bicarbonate
4. extractionextracted with ethyl acetate (3×5 ml)
5. washThe combined organic are washed with brine
6. dry with materialdried over magnesium sulfate
7. customPurification of the
8. concentrationconcentrate by preparative TLC on silica gel