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A nutrient medium (100 ml, pH 7.2) consisting of polypeptone (1.0%), yeast extract (1.0%) and glycerin (1.0%) were poured into an Erlenmeyer flask (500 ml) with a baffle and the flask was sterilized at 121° C. for 15 minuets. Previously, Pseudomonas sp. DS-K-436-1 were made stationary incubation in the agar medium (pH 7.2) containing polypeptone (1.0%), yeast extract (1.0%) and glycerin (1.0%) at 30° C. for 24 hours to prepare seed strains and a loopful of the strains was seeded to the above medium. The culture medium was cultivated with agitation (125 rpm) at 30° C. for 24 hours. After the cultivation, the culture broth was taken out, the cells were collected by centrifugation and washed three times by phosphate buffer (20 m M, pH 7.2) containing magnesium sulfate (2 mM) to prepare resting cells. The cells were suspended in the phosphate buffer containing 1.0% of calcium carbonate in an Erlenmeyer flask (500 ml) equipped with a baffle. To the suspension was added 1 ml of racemic 4-chloro-1,3-butanediol and the mixture was reacted at 30° C. under stirring. At that time the remaining amount of racemic 4-chloro-1,3-butanediol was measured with gas chromatography (column support: PEG20M, 60-80 mesh) to be 35% in the remaining ratio. After the reaction, the reaction mixture was concentrated to about 1 ml and extracted with ethanol. The extract was dried over magnesium sulfate and the solvent was removed in vacuo to give 368 mg of 4-chloro-1,3-butanediol and 590 mg of 1,2,4-butanetriol. The identification and determination of these compounds was made by subjecting to the above gas chromatography and GC-MS.

1. extractionextract (1.0%) and glycerin (1.0%)
2. additionwere poured into an Erlenmeyer flask (500 ml) with a baffle
3. customwas sterilized at 121° C. for 15 minuets
4. additioncontaining polypeptone (1.0%), yeast
5. extractionextract (1.0%) and glycerin (1.0%) at 30° C. for 24 hours
6. customto prepare seed strains
7. customthe cells were collected by centrifugation
8. washwashed three times by phosphate buffer (20 m M, pH 7.2)
9. additioncontaining magnesium sulfate (2 mM)
10. customto prepare
11. customequipped with a baffle
12. customthe mixture was reacted at 30° C.
13. customAfter the reaction
14. concentrationthe reaction mixture was concentrated to about 1 ml
15. extractionextracted with ethanol
16. dry with materialThe extract was dried over magnesium sulfate
17. customthe solvent was removed in vacuo