反应详情

In a 25 mL three-necked flask, (S)-dibenzyl 2-(2-(4-((2R,3S,4R,5S)-3-(3-chloro-2-fluorophenyl)-4-(4-chloro-2-fluorophenyl)-4-cyano-5-neopentylpyrrolidine-2-carboxamido)-3-methoxybenzoyloxy)acetamido)pentanedioate (Example 70, 61 mg, 62.0 μmol) was combined with isopropanol (5 mL) at room temperature. Then palladium on carbon (33.0 mg, 31.0 μmol) was added. The flask was flushed with nitrogen, and then replaced with hydrogen. It was stirred at room temperature under hydrogen balloon. After 30 min, the reaction mix was filtered through a Celite cake to remove the catalyst. The Celite cake was washed thoroughly with isopropanol. The filtrate and washing solutions were combined and concentrated to give a crude product, which was purified with reverse phase high-performance liquid chromatography. The pure fractions were concentrated and lyophilized to give (S)-2-[2-(4-{[(2R,3S,4R,5S)-4-(4-chloro-2-fluoro-phenyl)-3-(3-chloro-2-fluoro-phenyl)-4-cyano-5-(2,2-dimethyl-propyl)-pyrrolidine-2-carbonyl]-amino}-3-methoxy-benzoyloxy)-acetylamino]-pentanedioic acid as a white product (20 mg, 33% yield). LCMS (ES+) m/z calcd. for C38H39Cl2F2N4O9 [(M+H)+]: 803. found: 803.

1. customThe flask was flushed with nitrogen
2. additionthe reaction mix
3. filtrationwas filtered through a Celite cake
4. customto remove the catalyst
5. washThe Celite cake was washed thoroughly with isopropanol
6. washThe filtrate and washing solutions
7. concentrationconcentrated
8. customto give a crude product, which
9. customwas purified with reverse phase high-performance liquid chromatography
10. concentrationThe pure fractions were concentrated