1-Ethyl-3-(3'-dimethylaminopropyl)carbodiimide → 1-(Chloromethyl)-3-{5-[2-(dimethylamino)ethoxy]indol-2-carbonyl}-N-(2-hydroxyethyl)-5-nitro-1,2-dihydro-3H-benzo[e]indole-7-sulfonamide

HRID 2238380Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 2238380
[Cl:1][CH2:2][CH:3]1[C:11]2[C:10]3[CH:12]=[CH:13][C:14]([S:16]([NH:19][CH2:20][CH2:21][OH:22])(=[O:18])=[O:17])=[CH:15][C:9]=3[C:8]([N+:23]([O-:25])=[O:24])=[CH:7][C:6]=2[NH:5][CH2:4]1.Cl.[CH3:27][N:28]([CH3:44])[CH2:29][CH2:30][O:31][C:32]1[CH:33]=[C:34]2[C:38](=[CH:39][CH:40]=1)[NH:37][C:36]([C:41](O)=[O:42])=[CH:35]2.CCN=C=NCCCN(C)C>CC(N(C)C)=O>[Cl:1][CH2:2][CH:3]1[C:11]2[C:10]3[CH:12]=[CH:13][C:14]([S:16]([NH:19][CH2:20][CH2:21][OH:22])(=[O:17])=[O:18])=[CH:15][C:9]=3[C:8]([N+:23]([O-:25])=[O:24])=[CH:7][C:6]=2[N:5]([C:41]([C:36]2[NH:37][C:38]3[C:34]([CH:35]=2)=[CH:33][C:32]([O:31][CH2:30][CH2:29][N:28]([CH3:44])[CH3:27])=[CH:40][CH:39]=3)=[O:42])[CH2:4]1

Komponenten

5

Edukt 3

HCID 159081-Ethyl-3-(3'-dimethylaminopropyl)carbodiimideHCID 686908585-[2-(Dimethylamino)ethoxy]indole-2-carboxylic acid hydrochlorideHCID 686939191-(chloromethyl)-N-(2-hydroxyethyl)-5-nitro-1,2-dihydro-3H-benzo[e]indole-7-sulfonamide

Produkt 1

HCID 115273651-(Chloromethyl)-3-{5-[2-(dimethylamino)ethoxy]indol-2-carbonyl}-N-(2-hydroxyethyl)-5-nitro-1,2-dihydro-3H-benzo[e]indole-7-sulfonamideAusbeute 21 %

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 31374DimethylacetamideLösungsmittel

Versuchsvorschrift

(Scheme C). A mixture of compound 121 (46 mg, 0.11 mmol), 5-[2-(dimethylamino)ethoxy]indole-2-carboxylic acid hydrochloride (37 mg, 0.13 mmol) and EDCI (83 mg, 0.44 mmol) in DMA (3 mL) was stirred under a N2 atmosphere for 4 h. The mixture was then partitioned between CH2Cl2 and cold (0° C.) 5% aqueous KHCO3. The aqueous portion was extracted with cold CH2Cl2 (×4) and the combined extracts were washed with H2O (×2), brine (×2), dried and evaporated. The residue was dissolved in CH2Cl2/MeOH and the solvents were evaporated under reduced pressure until precipitation began. The precipitate was filtered off and washed with MeOH to give 35 (14 mg, 21%): mp 205-210° C.; 1H NMR [(CD3)2SO] δ 11.73 (s, 1H), 9.30 (s, 1H), 8.85 (d, J=1.6 Hz, 1H), 8.43 (d, J=8.9 Hz, 0.1H), 8.03 (dd, J=8.9, 1.6 Hz, 1H), 7.92 (t, J=5.4 Hz, 1H), 7.41 (d, J=8.9 Hz, 1H), 7.22 (d, J=1.4 Hz, 1H), 7.21 (d, J=2.3 Hz, 1H), 6.94 (dd, J=8.9, 2.4 Hz, 1H), 4.97 (t, J=10.3, Hz, 1H), 4.74 (dd, J=10.9, 2.3 Hz, 1H), 4.68-4.61 (m, 2H), 4.19-4.09 (m, 2H), 4.07 (t, J=5.9 Hz, 2H), 3.43-3.35 (m, 21H), 2.88 (q, J=5.8 Hz, 21H), 2.66 (t J=5.8 Hz, 2H), 224 (s, 6H). HRMS (FAB) calcd. for C28H3035ClN5O7S (MH+) m/z 616.1633, found 616.1630.

Aufarbeitung

  1. customThe mixture was then partitioned between CH2Cl2 and cold (0° C.) 5% aqueous KHCO3
  2. extractionThe aqueous portion was extracted with cold CH2Cl2 (×4)
  3. washthe combined extracts were washed with H2O (×2), brine (×2)
  4. customdried
  5. customevaporated
  6. dissolutionThe residue was dissolved in CH2Cl2/MeOH
  7. customthe solvents were evaporated under reduced pressure until precipitation
  8. filtrationThe precipitate was filtered off
  9. washwashed with MeOH

Quellen und Nachweise

ORD-Eintrag
ord-cf6781b9187d49288ab9b8c307639ad5
Quelldatensatz
uspto-grants-2010_05
Patentnummer
US07718688B2

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