反应详情

To a solution of 2,2,2-trichloro-1-{10-[(2′-methyl-1,1′-biphenyl-3-yl)carbonyl]-10,11-dihydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-3-yl}ethanone of Step C (0.600 g, 1.1 mmol) and dimethyl sulfoxide (0.41 mL, 5.8 mmol) in dry acetonitrile (5.5 mL) at room temperature under nitrogen was added 3-(aminomethyl)pyridine (0.23 mL, 2.2 mmol) and the reaction mixture heated to reflux for 45 hours. The cooled reaction mixture was concentrated in vacuo, the oily residue redissolved in ethyl acetate (25 mL), washed with 1 M sodium hydroxide (25 mL), water (25 mL) and brine (25 mL), dried over anhydrous magnesium sulfate, and concentrated in vacuo to give a pale orange foam. Purification by flash chromatography using ethyl acetate as solvent afforded a pale yellow foam that was recrystallized from ethyl acetate/diethyl ether/hexane to give the title compound (0.435 g, 74%) as an off-white crystalline solid.

1. temperaturethe reaction mixture heated
2. temperatureto reflux for 45 hours
3. customThe cooled reaction mixture
4. concentrationwas concentrated in vacuo
5. dissolutionthe oily residue redissolved in ethyl acetate (25 mL)
6. washwashed with 1 M sodium hydroxide (25 mL), water (25 mL) and brine (25 mL)
7. dry with materialdried over anhydrous magnesium sulfate
8. concentrationconcentrated in vacuo
9. customto give a pale orange foam
10. customPurification by flash chromatography
11. customafforded a pale yellow foam that
12. customwas recrystallized from ethyl acetate/diethyl ether/hexane