反应详情

温度

0 °C

1.20 g of N-{6-[(E)-2-(6-bromo-3H-imidazo[4,5-b]pyridin-2-yl)-vinyl]-4-methyl-pyridin-2-yl}-acetamide (compound B10) are suspended in 12 ml of aqueous sulphuric acid (10% strength) at 115° C. for 17 h. Subsequently, the mixture is cooled to 0° C. and conc. aqueous ammonia is added until pH=10. Thereafter, the mixture is extracted 3 times each with 70 ml of ethyl acetate. The combined organic layers are dried using sodium sulphate, filtered with suction, and evaporated to dryness to yield 880 mg of the title compound as an oil, which solidifies upon standing. M.p. 280° C. ESI-MS: 330.2/332.2 (MH+; 100%/90%). TLC: Rf=0.35 (dichloromethane/methanol 10:1).

1. extractionThereafter, the mixture is extracted 3 times each with 70 ml of ethyl acetate
2. customThe combined organic layers are dried
3. filtrationfiltered with suction
4. customevaporated to dryness